Part:BBa_K3882000:Design

Design Notes

T7 promoter is a high efficiency element which can have a high protein level of LUXR inside the BL21 E. coli bacteria. High level of LUXR expressed will makes the BL21 more sensitive to AHL in the environment.

Lac operator is an inhibitory DNA element which can shut down the function of AHL sensing by inhibit the transcription of LuxR gene. Thus, the AHL release by the BL21 E. coli can not be detected when culturing at the initial steps.

LuxR is the most widely used gene in the quorum system. We use the LUXR protein to capture the AHL in the environment released by P. aeruginosa.

T7 terminator is a commonly used as a transcriptional stop region for T7 promoter and QS promoter.

QS promoter is the original sequences from Vibrio fischeri which controls the expression of Lux IABC gene expression.

mCherry gene is the commonly used as a report to show the frequency of the QS promoter.

Source

QS promotor is the sequence from Vibrio fischeri. The paper published by T. 0. Baldwin, J. H. Devine, R. C. Heckel, J.-W. Lin, and G. S. Shadel Department of Biochemistry and Biophysics, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843, USA

References

T. 0. Baldwin, J. H. Devine, R. C. Heckel, J.-W. Lin, and G. S. Shadel Department of Biochemistry and Biophysics, Texas A&M University, and Texas Agricultural Experiment Station, College Station, Texas 77843, USA